Recombinant Human ICAM1 protein, His-tagged

• Cat.No.: ICAM1-2364H
• Product Overview: Recombinant Human ICAM1 protein(AAH15969)(Gln28-Glu480), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Gln28-Glu480
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ICAM-1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ICAM1 intercellular adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: ICAM1
• Synonyms: ICAM1; intercellular adhesion molecule 1; BB2; CD54; human rhinovirus receptor; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; P3.58
• Gene ID: 3383
• mRNA Refseq: NM_000201
• Protein Refseq: NP_000192
• MIM: 147840
• UniProt ID: P05362