Recombinant Human Hypoxanthine Phosphoribosyltransferase 1, His-tagged

• Cat.No.: HPRT1-1796H
• Product Overview: Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALD
• Molecular Mass: 26.7 kDa (238aa), confirmed by MALDI-TOF
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HPRT1 hypoxanthine phosphoribosyltransferase 1 [ Homo sapiens ]
• Official Symbol: HPRT1
• Synonyms: HPRT1; hypoxanthine phosphoribosyltransferase 1; HPRT; HGPRT; hypoxanthine-guanine phosphoribosyltransferase; HGPRTase; EC 2.4.2.8; Lesch-Nyhan syndrome
• Gene ID: 3251
• mRNA Refseq: NM_000194
• Protein Refseq: NP_000185
• MIM: 308000
• UniProt ID: P00492
• Chromosome Location: Xq26.1
• Pathway: Drug metabolism - other enzymes; Metabolic pathways; Purine metabolism
• Function: hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; protein binding; protein homodimerization activity; transferase activity, transferring glycosyl groups