Recombinant Human HSPA5, His-tagged

• Cat.No.: HSPA5-27412TH
• Product Overview: Recombinant full length human GRP78 BiP Protein with His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.
• Conjugation: HIS
• Biological activity: HSPA5-27412TH has ATPase activity at the time of manufacture of 2.3μM phosphate liberated/hr/μg protein in a 200μl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: 120mM ImidazoleConstituents: 10% Glycerol, 0.15M Sodium chloride, 20mM Sodium phosphate, pH 7.4
• Storage: Store at -20°C. Stable for 12 months at -20°C
• Sequence Similarities: Belongs to the heat shock protein 70 family.
• Full Length: Full L.

Gene Information

• Gene Name: HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) [ Homo sapiens ]
• Official Symbol: HSPA5
• Synonyms: HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); GRP78, heat shock 70kD protein 5 (glucose regulated protein, 78kD); 78 kDa glucose-regulated protein; BiP
• Gene ID: 3309
• mRNA Refseq: NM_005347
• Protein Refseq: NP_005338
• MIM: 138120
• Uniprot ID: P11021
• Chromosome Location: 9q33.3
• Pathway: Activation of Chaperones by ATF6-alpha, organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem
• Function: ATP binding; ATPase activity; calcium ion binding; chaperone binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process