Recombinant Human HNRNPF Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HNRNPF-1603H
• Product Overview: HNRNPF MS Standard C13 and N15-labeled recombinant protein (NP_001091676) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 45.7 kDa
• AA Sequence: MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HNRNPF heterogeneous nuclear ribonucleoprotein F [ Homo sapiens (human) ]
• Official Symbol: HNRNPF
• Synonyms: HNRNPF; heterogeneous nuclear ribonucleoprotein F; HNRPF; HnRNP F protein; nucleolin-like protein mcs94-1; mcs94-1; OK/SW-cl.23; MGC110997
• Gene ID: 3185
• mRNA Refseq: NM_001098206
• Protein Refseq: NP_001091676
• MIM: 601037
• UniProt ID: P52597