Recombinant Human HLA-A protein, His-Avi-tagged

• Cat.No.: HLA-A-069H
• Product Overview: Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein(0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL)(Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide) is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
  > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891