Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer protein, His-Avi-tagged, Biotinylated
Cat.No. : | HLA-A&B2M&WT-1-4432H |
Product Overview : | Recombinant Biotinylated Human WT-1 (HLA-A*02:01) Protein(Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide) is expressed from HEK293 with His tag and Avi tag at the C-terminal. |
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Species : | Human |
Source : | HEK293 |
Tag : | His&Avi |
ProteinLength : | Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and RMFPNAPYL peptide |
Tag : | C-His-Avi |
Conjugation/Label : | Biotin |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Storage : | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
GeneID 2 : | 567 |
Gene Name | HLA-A major histocompatibility complex, class I, A [ Homo sapiens ] |
Official Symbol | HLA-A |
Synonyms | HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655; |
Gene ID | 3105 |
mRNA Refseq | NM_001242758 |
Protein Refseq | NP_001229687 |
UniProt ID | P01891 |
Gene Name 2 | B2M beta-2-microglobulin [ Homo sapiens ] |
Official Symbol 2 | B2M |
Synonyms 2 | B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules |
mRNA Refseq 2 | NM_004048 |
Protein Refseq 2 | NP_004039 |
MIM 2 | 109700 |
UniProt ID 2 | P61769 |
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Not For Human Consumption!
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