Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer protein, His-Avi-tagged

• Cat.No.: HLA-A&B2M&NY-ESO-1-5632H
• Product Overview: Recombinant Human NY-ESO-1 (HLA-A*02:01) Protein(Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide) is expressed from HEK293 with His tag and Avi tag at the C-terminal.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and SLLMWITQC peptide
• Tag: C-His-Avi
• Form: Supplied as 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4).
• Bio-activity: Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 8.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneID 2: 567

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891
• Gene Name 2: B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol 2: B2M
• Synonyms 2: B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• mRNA Refseq 2: NM_004048
• Protein Refseq 2: NP_004039
• MIM 2: 109700
• UniProt ID 2: P61769