Recombinant Human HK1, His-Twin Strep-tagged

• Cat.No.: HK1-177H
• Product Overview: Recombinant Human HK1 /Hexokinase-1 Protein,C-Twin Strep & His Tag (rh HK1 /Hexokinase-1,Twin Strep) Asp 16 - Ser 917 (Accession # NP_000179) was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His&Twin Strep
• Protein Length: 16-917 a.a.
• Description: Hexokinase-1 (HK1) is also known as Brain form hexokinase, Hexokinase type I (HK I). Hexokinase-1 is a monomer which can interact with RABL2/RABL2A. HK1 binds preferentially to GTP-bound RABL2 By similarity.HK1 interacts with VDAC1 and the HK1-VDAC1 complex interacts with ATF2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway.
• Predicted N Terminal: Asp 16
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 and some stabilizers. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: rh HK1 /Hexokinase-1,Twin Strep is fused with polyhistidine tagged Twin Strep Tag at the C-terminus, and has a calculated MW of 105 kDa. The predicted N-terminus is Asp 16. DTT-reduced Protein migrates as 95-115 kDa in SDS-PAGE.
• Endotoxin: Less than 1.0 EU per μg of the rh HK1 /Hexokinase-1,Twin Strep by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Applications: Recombinant Proteins
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Gene Information

• Gene Name: HK1 hexokinase 1 [ Homo sapiens ]
• Official Symbol: HK1
• Synonyms: HK1; hexokinase 1; hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
• Gene ID: 3098
• mRNA Refseq: NM_000188
• Protein Refseq: NP_000179
• MIM: 142600
• UniProt ID: P19367
• Chromosome Location: 10q22
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Butirosin and neomycin biosynthesis, organism-specific biosystem; Butirosin and neomycin biosynthesis, conserved biosystem; Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Fructose and mannose metabolism, organism-specific biosystem
• Function: ATP binding; hexokinase activity; kinase activity; nucleotide binding; transferase activity