Recombinant Human HIKESHI Protein, MYC/DDK-tagged

• Cat.No.: HIKESHI-240H
• Product Overview: Recombinant Human HIKESHI fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 21.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: HIKESHI Hikeshi, heat shock protein nuclear import factor [ Homo sapiens ]
• Official Symbol: HIKESHI
• Synonyms: HLD13; L7RN6; OPI10; HSPC138; HSPC179; C11orf73
• Gene ID: 51501
• mRNA Refseq: NM_016401
• Protein Refseq: NP_057485
• MIM: 614908
• UniProt ID: Q53FT3