Active Recombinant Human HHLA2 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: HHLA2-551H
• Product Overview: Biotinylated Human B7-H7, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 23-344 a.a.
• Predicted N Terminal: Ile 23
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human CD28H, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-H7, Fc Tag, Avi Tag with a linear range of 5-78 ug/mL.
• Molecular Mass: The protein has a calculated MW of 65.9 kDa. The protein migrates as 80-90 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.45-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HHLA2 HERV-H LTR-associating 2 [ Homo sapiens ]
• Official Symbol: HHLA2
• Synonyms: HHLA2; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; human endogenous retrovirus-H long terminal repeat-associating protein 2
• Gene ID: 11148
• mRNA Refseq: NM_007072
• Protein Refseq: NP_009003
• MIM: 604371
• UniProt ID: Q9UM44