Recombinant Human HFE2 protein(Met1-Ser399), His-tagged

• Cat.No.: HFE2-7288H
• Product Overview: Recombinant Human HFE2 isoform a (Q6ZVN8-1) (Met 1-Ser 399) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: Met1-Ser399
• Form: Lyophilized from sterile PBS, 500mM NaCl, pH 7.0, 10% gly Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human HFE2 consists of 374 amino acids and predicts a molecular mass of 40 kDa. As a result of intracellular cleavage, the apparent molecular mass of the protein is approximately 20, 34 and 44 KDa in SDS-PAGE under reducing conditions, corresponding to the N-terminal, C-terminal portions and the full-length respectively.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HFE2 hemochromatosis type 2 (juvenile) [ Homo sapiens ]
• Official Symbol: HFE2
• Synonyms: HFE2; hemochromatosis type 2 (juvenile); hemojuvelin; haemojuvelin; HFE2A; HJV; JH; repulsive guidance molecule c; RGMC; RGM domain family member C; hemochromatosis type 2 protein; MGC23953
• Gene ID: 148738
• mRNA Refseq: NM_145277
• Protein Refseq: NP_660320
• MIM: 608374
• UniProt ID: Q6ZVN8