Recombinant Human Hexokinase 3, His-tagged

• Cat.No.: HK3-1143H
• Product Overview: Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexokinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: MGSSHHHHHH SSGLVPRGSH MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ LLRDAIRRQG AY
• Molecular Mass: 101.1 kDa (943aa)
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HK3 hexokinase 3 (white cell) [ Homo sapiens ]
• Official Symbol: HK3
• Synonyms: Hexokinase-3; EC 2.7.1.1; Hexokinase type III; HK III; HXK3; HK3; ATP:D-hexose 6-phosphotransferase; hexokinase 3; hexokinase 3 (white cell); HK-3
• Gene ID: 3101
• mRNA Refseq: NM_002115
• Protein Refseq: NP_002106
• MIM: 142570
• UniProt ID: P52790
• Chromosome Location: 5q35.2
• Pathway: Aminosugars metabolism; Fructose and mannose metabolism; Galactose metabolism; Glycolysis / Gluconeogenesis; Starch and sucrose metabolism; Streptomycin biosynthesis; Metabolism of carbohydrates
• Function: ATP binding; hexokinase activity; kinase activity; nucleotide binding; transferase activity