Recombinant Human Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1

• Cat.No.: HSP90AA1-318H
• Product Overview: Recombinant human HSP90AA1, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques (752 aa, 86.8 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: HSP 90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.4) containing 100 mM NaCl.
• Molecular Weight: 86.8kDa (752aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLED PQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HSP90AA1
• Synonyms: HSP90AA1; heat shock protein 90kDa alpha (cytosolic), class A member 1; Hsp90 α; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; FLJ31884; HSP90AA1; Renal carcinoma antigen NY-REN-38; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1.
• Gene ID: 3320
• mRNA Refseq: NM_001017963.2
• Protein Refseq: NP_001017963.2
• MIM: 140571
• UniProt ID: Q86SX1
• Chromosome Location: 14q32.33
• Pathway: Antigen processing and presentation; Prostate cancer; Cell Cycle, Mitotic; Metabolism of nitric oxide.
• Function: ATP binding; TPR domain binding; TPR domain binding; nitric-oxide synthase regulator activity; nucleotide binding; protein homodimerization activity; unfolded protein binding.