Recombinant Human HDDC3, His-tagged

• Cat.No.: HDDC3-1528H
• Product Overview: Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: HDDC3, also known as guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Mass: 17.9 kDa (160aa), confirmed by MALDI-TOF
• Protein length: 1-140aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HDDC3 HD domain containing 3 [ Homo sapiens (human) ]
• Official Symbol: HDDC3
• Synonyms: HDDC3; MESH1; MGC45386; guanosine-3",5"-bis(diphosphate); 3"-pyrophosphohydrolase MESH1; (ppGpp)ase; OTTHUMP00000180560; OTTHUMP00000180561; metazoan SpoT homolog 1; metazoan SpoT homolog-1; HD domain-containing protein 3; penta-phosphate guanosine-3"-pyrophosphohydrolase
• Gene ID: 374659
• mRNA Refseq: NM_198527
• Protein Refseq: NP_940929
• UniProt ID: Q8N4P3
• Chromosome Location: 15q26.1
• Pathway: Purine metabolism
• Function: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; metal ion binding