Recombinant Human HDAC3 Protein, His-tagged

• Cat.No.: HDAC3-2235H
• Product Overview: Recombinant Human HDAC3 Protein (Met1-Ile428) with a N-His tag was expressed in E. coli.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 52.5 kDa
  Accurate Molecular Mass: 53 kDa
• Protein length: Met1-Ile428
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens (human) ]
• Official Symbol: HDAC3
• Synonyms: HDAC3; histone deacetylase 3; HD3; RPD3; RPD3 2; SMAP45; RPD3-2
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379