Recombinant Human Guanine Nucleotide Binding Protein (G protein), Alpha Inhibiting Activity Polypeptide 1, His-tagged

• Cat.No.: GNAI1-6873H
• Product Overview: Recombinant human GNAI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 42.7 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-354aa
• Description: GNAI1, also known as guanine nucleotide-binding protein G(i) subunit alpha, functions to relay information from cell surface receptors to intracellular effectors. Also this protein functions to open atrial potassium channels.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Molecular Mass: 42.7 kDa (377aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAREVKLL LLGAGESGKS TIVKQMKIIH EAGYSEEECK QYKAVVYSNT IQSIIAIIRA MGRLKIDFGD SARADDARQL FVLAGAAEEG FMTAELAGVI KRLWKDSGVQ ACFNRSREYQ LNDSAAYYLN DLDRIAQPNY IPTQQDVLRT RVKTTGIVET HFTFKDLHFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTDTSIILF LNKKDLFEEK IKKSPLTICY PEYAGSNTYE EAAAYIQCQF EDLNKRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKDCGLF
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 [ Homo sapiens ]
• Official Symbol: GNAI1
• Synonyms: GNAI1; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein; Gi
• Gene ID: 2770
• mRNA Refseq: NM_001256414
• Protein Refseq: NP_001243343
• MIM: 139310
• UniProt ID: P63096
• Chromosome Location: 7q21-q22
• Pathway: ADP signalling through P2Y purinoceptor 12, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Adenylate cyclase inhibitory pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled serotonin receptor binding; GTP binding; GTPase activity; GTPase activity; GTPase activity; guanyl nucleotide binding; guanyl-nucleotide exchange factor activity; metal ion binding; nucleotide binding; phosphatidylinositol phospholipase C activity; protein binding; signal transducer activity