Recombinant Human GPT, His-tagged

• Cat.No.: GPT-26390TH
• Product Overview: Recombinant full-length Human Alanine Transaminase with a N terminal His tag. 516 amino acids with a predicted MWt 56.8 kDa including tag.

Specification

• Description: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
• Protein length: 496 amino acids
• Conjugation: HIS
• Molecular Weight: 56.800kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 20% Glycerol, 0.03% DTT
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMASSTGDRSQAVRHGLRAKV LTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKK PFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSP NFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDV ARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEG HTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAW ALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTREC IEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKV LMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNM DAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDP SFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQG AMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETG ICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFH AKFTLEYS
• Sequence Similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Gene Information

• Gene Name: GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Homo sapiens ]
• Official Symbol: GPT
• Synonyms: GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1
• Gene ID: 2875
• mRNA Refseq: NM_005309
• Protein Refseq: NP_005300
• MIM: 138200
• Uniprot ID: P24298
• Chromosome Location: 8q24.2-qter
• Pathway: Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: 1-aminocyclopropane-1-carboxylate synthase activity; L-alanine:2-oxoglutarate aminotransferase activity; L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity