Recombinant Human GPI Protein, GST-tagged

• Cat.No.: GPI-3928H
• Product Overview: Recombinant Human GPI Protein (Ala2-Gln558) is produced by E. Coli expression system. This protein is fused with a GST tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: Ala2-Gln558
• Description: Glucose-6-phosphate isomerase (GPI), is also known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI), Sperm antigen 36 (SA-36). GPI belongs to the GPI family. GPI is homodimer in the catalytically active form and is monomer in the secreted form. GPI can catalyze D-glucose 6-phosphate to be D-fructose 6-phosphate. Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
• Form: Protein in [50 mM Tris, pH7.5 with 10 mM GSH, 1 mM EDTA] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
• Molecular Mass: 86.8 kDa
• Endotoxin: < 1.0 EU/ug by LAL method
• Purity: >95% as determined by SDS-PAGE
• Notes: All products are For Research Use Only, and not for diagnostic or therapeutic purposes.
• Storage: Lyophilized form is stable at room temperature for 3 weeks. Keep at -20 centigrade for long term storage. After reconstitution, store at 4 centigrade for 2-7 days or at below -20 centigrade for up to 3 months.
• Reconstitution: Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
• Shipping: Ship at ambient conditions

Gene Information

• Gene Name: GPI glucose-6-phosphate isomerase [ Homo sapiens ]
• Official Symbol: GPI
• Synonyms: GPI; AMF; NLK; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; PGI; PHI; GNPI; SA36; SA-36; DKFZp686C13233
• Gene ID: 2821
• mRNA Refseq: NM_000175
• Protein Refseq: NP_000166
• MIM: 172400
• UniProt ID: P06744