Recombinant Human GPD1 protein(Met1-Met349), His-tagged

• Cat.No.: GPD1-281H
• Product Overview: Recombinant Human GPD1(P21695) (Met1-Met349) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Met1-Met349
• Form: Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to catalyze dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate, The specific activity is > 400 pmols/min/ug.
• Molecular Mass: The recombinant human GPD1 consists of 364 amino acids and predicts a molecular mass of 39.4 KDa. It migrates as an approximately 33-37 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPD1 glycerol-3-phosphate dehydrogenase 1 (soluble) [ Homo sapiens ]
• Official Symbol: GPD1
• Synonyms: GPD1; glycerol-3-phosphate dehydrogenase 1 (soluble); glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerophosphate dehydrogenase; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; GPD-C; HTGTI; GPDH-C; FLJ26652
• Gene ID: 2819
• mRNA Refseq: NM_001257199
• Protein Refseq: NP_001244128
• MIM: 138420
• UniProt ID: P21695