Recombinant Human GPA33 protein, His-Avi-tagged, Biotinylated

• Cat.No.: GPA33-795HB
• Product Overview: Biotinylated Recombinant Human GPA33 protein(Q99795-1)(Ile22-Val235), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ile22-Val235
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GPA33 glycoprotein A33 (transmembrane) [ Homo sapiens ]
• Official Symbol: GPA33
• Synonyms: GPA33; glycoprotein A33 (transmembrane); cell surface A33 antigen; A33; transmembrane glycoprotein A33; MGC129986; MGC129987
• Gene ID: 10223
• mRNA Refseq: NM_005814
• Protein Refseq: NP_005805
• MIM: 602171
• UniProt ID: Q99795