Recombinant Human glypican 4, His-tagged

• Cat.No.: GPC4-519H
• Product Overview: GPC4, 401-529aa Human, His tag, E.coli

Specification

• Description: Glypican 4, also known as GPC4, is a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. GPC4 is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Recombinant human GPC4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Molecular Mass: 18 kDa (165aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSSLP SNVCNDERMA AGNGNEDDCW NGKGKSRYLF AVTGNGLANQ GNNPEVQVDT SKPDILILRQ IMALRVMTSK MKNAYNGNDV DFFDISDESS GEGSGSGCEY QQCPSEFDYN ATDHAGKSAN EKADS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Protein length: 401-529 a.a.

Gene Information

• Gene Name: GPC4 glypican 4 [ Homo sapiens ]
• Official Symbol: GPC4
• Synonyms: GPC4; glypican 4; glypican-4; glypican proteoglycan 4; K glypican; dJ900E8.1 (glypican 4); K-glypican
• Gene ID: 2239
• mRNA Refseq: NM_001448
• Protein Refseq: NP_001439
• MIM: 300168
• UniProt ID: O75487
• Chromosome Location: Xq26.1
• Pathway: Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem
• Function: heparan sulfate proteoglycan binding