Recombinant Human Glucose-6-phosphate Dehydrogenase, His-tagged

• Cat.No.: G6PD-2397H
• Product Overview: Recombinant humanG6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cellusing baculovirus expression system and purified by using conventionalchromatography

Specification

• Cat. No.: G6PD-2397H
• Description: Glucose-6-phosphatedehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphatepathway, a metabolic pathway that supplies reducing energy to cells bymaintaining the level of NADPH. G6PD converts glucose-6-phosphate into6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH inturn maintains the level of glutathione in these cells that helps protect thered blood cells against oxidative damage. G6PD deficiency cause acutehemolytic anemia
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol 0.1mM PMSF, 2mM EDTA, 2mMDTT, 200mM NaCl
• Molecular Weight: 61.4kDa (535aa)
• Purity: > 95% by SDS -PAGE
• Concentration: 0.5mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGLLPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHLGSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRIDHYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCLVAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAVVLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPGMFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPIPYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
• Biological activity: Specific activity is > 7 units/mlobtained by measuring the increase of NADPH in absorbance at 340 nm resultingfrom the reduction of NAD or NADP. One unit oxidizes 1.0 umoleD-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence ofbeta-NADP at pH 7.4 at 25C
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Officialsymbol: G6PD

Gene Information

• Gene Name: G6PD glucose-6-phosphatedehydrogenase [Homo sapiens]
• Synonyms: glucose-6-phosphatedehydrogenase; G6PD1; EC 1.1.1.49; OTTHUMP00000196180; glucose-6-phosphate1-dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD; OTTHUMP00000026034;OTTHUMP00000026039
• Gene ID: 2539
• mRNA Refseq: NM_001042351
• Protein Refseq: NP_001035810
• MIM: 305900
• UniProt ID: P11413
• Chromosome Location: Xq28
• Pathway: Glutathionemetabolism; Glutathione metabolism; Glutathione metabolism; Metabolicpathways; Metabolism of carbohydrates; Pentose Phosphate Pathway
• Function: NADPbinding; binding; glucose binding; glucose binding; glucose-6-phosphatedehydrogenase activity; oxidoreductase activity; protein homodimerizationactivity