Recombinant Human GLS protein, His & T7-tagged

• Cat.No.: GLS-1607H
• Product Overview: Recombinant Human GLS aa. (Ile221~Leu533 (Accession # O94925)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ile221~Leu533
• Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.9

Gene Information

• Gene Name: GLS glutaminase [ Homo sapiens (human) ]
• Official Symbol: GLS
• Synonyms: GLS; glutaminase; GAC; GAM; KGA; GLS1; AAD20; glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase; glutaminase C; glutaminase, phosphate-activated
• Gene ID: 2744
• mRNA Refseq: NM_001256310.1
• Protein Refseq: NP_001243239.1
• UniProt ID: O94925