Recombinant Human GET4, His-tagged

• Cat.No.: GET4-5063H
• Product Overview: Recombinant human GET4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 38.9 kDa (350aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD
• Purity: >90 % by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GET4 golgi to ER traffic protein 4 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: GET4
• Synonyms: GET4; golgi to ER traffic protein 4 homolog (S. cerevisiae); C7orf20, chromosome 7 open reading frame 20; Golgi to ER traffic protein 4 homolog; CEE; CGI 20; CGI 20 protein; conserved edge protein; H_NH1244M04.5; transmembrane domain recognition complex; 35kDa; TRC35; conserved edge expressed protein; conserved edge-expressed protein; transmembrane domain recognition complex, 35kDa; transmembrane domain recognition complex 35 kDa subunit; CGI-20; C7orf20
• Gene ID: 51608
• mRNA Refseq: NM_015949
• Protein Refseq: NP_057033
• MIM: 612056
• UniProt ID: Q7L5D6
• Chromosome Location: 7p22.3