Recombinant Human GBA protein, His-tagged

• Cat.No.: GBA-7841H
• Product Overview: Recombinant Human GBA aa. (Val334~Val498 (Accession # P04062)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val334~Val498
• Description: This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.1kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GBA glucosylceramidase beta [ Homo sapiens (human) ]
• Official Symbol: GBA
• Synonyms: Thbs4; thrombospondin 4; thrombospondin-4
• Gene ID: 2629
• mRNA Refseq: NM_000157.3
• Protein Refseq: NP_000148.2
• UniProt ID: P04062