Recombinant Human GAL3ST1 protein, His & GST-tagged

• Cat.No.: GAL3ST1-1615H
• Product Overview: Recombinant Human GAL3ST1 aa. (Leu57~Phe196 (Accession # Q99999)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. This gene encodes galactosylceramide sulfotransferase, which catalyzes the sulfation of membrane glycolipids including the final step in the synthesis of sulfatide, a major lipid component of the myelin sheath. This gene exhibits elevated expression in ovarian epithelial carcinoma and the encoded enzyme exhibits elevated activity in renal cell carcinoma. Mutations in this gene may be associated with reduced insulin resistance. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 48.5kDa
• Protein length: Leu57~Phe196
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.1

Gene Information

• Gene Name: GAL3ST1 galactose-3-O-sulfotransferase 1 [ Homo sapiens (human) ]
• Official Symbol: GAL3ST1
• Synonyms: GAL3ST1; galactose-3-O-sulfotransferase 1; CST; galactosylceramide sulfotransferase; 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; cerebroside (3'-phosphoadenylylsulfate:galactosylceramide 3') sulfotransferase
• Gene ID: 9514
• mRNA Refseq: NM_001318103.1
• Protein Refseq: NP_001305032.1
• UniProt ID: Q99999