Recombinant Human GAD67 protein(Met1-Leu594), His-tagged

• Cat.No.: GAD1-1180H
• Product Overview: Recombinant Human GAD1 (Q99259-1) (Met 1-Leu 594) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: Met1-Leu594
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human GAD1 consists of 605 amino acids and predicts a molecular mass of 68.3 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.