Recombinant Human Fms-Related Tyrosine Kinase 3 Ligand, His-tagged

• Cat.No.: FLT3LG-5423H
• Product Overview: Recombinant human FLT3LG contains 155amino acids and a 14 amino acid Histidine-based tag for a total length of 169amino acids. The predicted molecular weight of the recombinant FLT3LG is 19.9kDa, but the recombinant protein migrates as two bands with an apparentmolecular mass of 20 and 22 kDa in SDS-PAGE as a result of glycosylation.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: His
• Description: FLT3LGis a protein which in humans is encoded by the FLT3LG gene.FLT3LG is ahematopoietic four helical bundle cytokine. It is structurally homologous tostem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergywith other growth factors, FlT3LG stimulates the proliferation anddifferentiation of various blood cell progenitors.
• Form: Lyophilized from a0.2 um sterile filtered solution in 10 mM acetic acid, pH 5.0.
• Molecular Weight: 20and 22 kDa
• Purity: > 98%as determined by SDS-PAGE analysis. Purified product carries no pyrogenic or pro-inflammatorycontaminants, as assayed with monocyte activation test using Human 10-plexCytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
• EndotoxinLevel: < 0.005 ng/ug of FLT3LG
• BiologicalActivity: Determined by CellProliferation Assay for the dose-dependent effects of serial dilutions ofrecombinant human FLT3LG on AML5 cells. The ED50 is typically inthe range of 0.9-1.4 ng/ml.
• SpecificActivity: 1.11 x 106units/mg
• Reconstitution: Centrifuge vial prior to opening. Add sterile PBS to the vial tofully solubilize the protein to a concentration of 10 ug/ml.
• Storage: Store lyophilized protein at -20°Cand reconstituted protein in working aliquots at -20°C with a carrier protein(0.1% HSA or BSA) as a stabilizer for long term storage. Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
• OfficialSymbol: FLT3LG

Gene Information

• Gene Name: FLT3LGfms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Synonyms: FLT3LG; fms-relatedtyrosine kinase 3 ligand; FL; FLT3L; flt3 ligand; SL cytokine; FLt3L
• Gene ID: 2323
• mRNA Refseq: NM_001459
• Protein Refseq: NP_001450
• MIM: 600007
• UniProt ID: P49771
• Chromosome Location: 19q13.3
• Pathway: Cytokine-cytokine receptorinteraction; Hematopoietic cell lineage; Pathways in cancer
• Function: cytokine activity; receptor binding