Recombinant Human FLI1 protein, His & T7-tagged

• Cat.No.: FLI1-7818H
• Product Overview: Recombinant Human FLI1 aa. (Met1~Glu196 (Accession # Q01543)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.9kDa.
• Protein length: Met1~Glu196
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.0.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: FLI1 Fli-1 proto-oncogene, ETS transcription factor [ Homo sapiens (human) ]
• Official Symbol: FLI1
• Synonyms: Tpsb2; tryptase beta 2; Mcpt6; tryptase beta-2; mast cell protease 6; rMCP-6; tryptase-2
• Gene ID: 2313
• mRNA Refseq: NM_001167681.2
• Protein Refseq: NP_001161153.1
• UniProt ID: Q01543