Recombinant Human Fibroblast Growth Factor 5

• Cat.No.: FGF5-172H
• Product Overview: This recombinant human FGF-5 is a 27.6 kDa protein containing 252 amino acid residues and it was expressed inE.Coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Fibroblast Growth Factor-5 (FGF-5) , is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors.
• Supplied: Sterile filtered and the the protein was lyophilized from 2X PBS pH7.2( no additives).
• Purity: >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-5.
• Reconstitution: The lyophilized FGF-5 should be reconstituted in 1X PBS pH7.2 to a concentration of 1ug/ul (1mg/ml). Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4°Cfor up to 1 week or stored at - 20°Cfor future use. For most in-vitro applications, human FGF-5 exerts its biological activity in the concentration range of 0.1 to 20ng/ml.
• Note: If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml.
• Reactivity: The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <1ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg.
• Amino Acid Sequence: MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAASL GSQGSGLEQS SFQWSPSGR RTGSLYCRVG IGFHLQIYPDG KVNGSHEANM LSVLEIFAVSQ GIVGIRGVFSN KFLAMSKKGK LHASAKFTDD CKFRERFQEN SYNTYASAIHR TEKTGREWYV ALNKRGKAKR GCSPRVKPQH ISTHFLPRFK QSEQPE LSFTV TVPEK KNPPSP IKSK IPLSAPRKNT NSVKYRLKFRFG
• Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0°C. Reconstituted human FGF-5 should be stored in working aliquots at -20℃.

Gene Information

• Gene Name: FGF5 fibroblast growth factor 5 [ Homo sapiens ]
• Synonyms: FGF5; fibroblast growth factor 5; HBGF-5; Smag-82; heparin-binding growth factor 5; FGF-5; Heparin-binding growth factor 5
• Gene ID: 2250
• mRNA Refseq: NM_004464
• Protein Refseq: NP_004455
• MIM: 165190
• UniProt ID: P12034
• Chromosome Location: 4q21
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity