Recombinant Human FH, His-tagged

• Cat.No.: FH-165H
• Product Overview: Recombinant Human Fumarase /FH Protein (rh Fumarase /FH) Ala 45 - Lys 510 (Accession # AAH03108) was produced in E.coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 45-510 a.a.
• Description: Fumarate hydratase, mitochondrial is also known as Fumarase or FH, which belongs to the class-II fumarase/aspartase family and Fumarase subfamily. Fumarase is a homotetramer protein which is expressed in red blood cells. There are two substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. Fumarase also acts as a tumor suppressor.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: rh Fumarase /FH is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 51 kDa. The predicted N-terminus is Ala 45. DTT-reduced Protein migrates as 47 kDa in SDS-PAGE.
• Endotoxin: Less than 1.0 EU per μg of the rh Fumarase /FH by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: FH fumarate hydratase [ Homo sapiens ]
• Official Symbol: FH
• Synonyms: FH; fumarate hydratase; fumarate hydratase, mitochondrial; fumarase; MCL; LRCC; HLRCC; MCUL1
• Gene ID: 2271
• mRNA Refseq: NM_000143
• Protein Refseq: NP_000134
• MIM: 136850
• UniProt ID: P07954
• Chromosome Location: 1q42.1
• Pathway: Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, second carbon oxidation, 2-oxoglutarate =>oxaloacetate, organism-specific biosystem; Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, conserved biosystem; Citric acid cycle (TCA cycle), organism-specific biosystem
• Function: fumarate hydratase activity; lyase activity