Recombinant Human FGF21 protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: FGF21-1567H
• Product Overview: Biotinylated Recombinant Human FGF21 protein(Q9NSA1)(His29-Ser209), fused with N-terminal mFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: N-mFc-Avi
• Tag: Non
• Protein Length: His29-Ser209
• Tag: N-mFc-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human FGF21, mFc Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FGF21 fibroblast growth factor 21 [ Homo sapiens ]
• Official Symbol: FGF21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 26291
• mRNA Refseq: NM_019113
• Protein Refseq: NP_061986
• MIM: 609436
• UniProt ID: Q9NSA1