Recombinant Human FEN1 Protein, His-tagged

• Cat.No.: FEN1-1477H
• Product Overview: Recombinant Human FEN1 Protein (Met1-Thr195) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Thr195
• Description: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.2 kDa
  Accurate Molecular Mass: 26 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FEN1 flap structure-specific endonuclease 1 [ Homo sapiens (human) ]
• Official Symbol: FEN1
• Synonyms: FEN1; flap structure-specific endonuclease 1; RAD2; flap endonuclease 1; DNase IV; FEN 1; maturation factor 1; MF1; maturation factor-1; FEN-1
• Gene ID: 2237
• mRNA Refseq: NM_004111
• Protein Refseq: NP_004102
• MIM: 600393
• UniProt ID: P39748