Recombinant Human FCGR2A protein, His-Avi-tagged

• Cat.No.: FCGR2A-5733H
• Product Overview: Recombinant Human FCGR2A protein(P12318-1)(Ala36-Ile218(R167)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ala36-Ile218(R167)
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.89 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
• Official Symbol: FCGR2A
• Synonyms: FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032
• Gene ID: 2212
• mRNA Refseq: NM_001136219
• Protein Refseq: NP_001129691
• MIM: 146790
• UniProt ID: P12318