Recombinant Human FCGR1A protein(Met1-Pro288), His-tagged

• Cat.No.: FCGR1A-2224H
• Product Overview: Recombinant Human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: 1-288 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Rituximab at 1 μg/mL can bind Human FcγRI / CD64 recombinant protein, with a linear range of 0.5-4.0 ng/mL. 2. Captured Human FcγRI / CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200). 3. Captured Human FcγRI / CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200). 4. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (ForteBio Octet Red384) . 5. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (ForteBio Octet Red384). 6. Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein with an affinity constant of 0.38 nM as determined in a BLI assay (ForteBio Octet Red384).
• Molecular Mass: The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
• Official Symbol: FCGR1A
• Synonyms: FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; CD64; CD64A; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; FCRI; IGFR1; FLJ18345
• Gene ID: 2209
• mRNA Refseq: NM_000566
• Protein Refseq: NP_000557
• MIM: 146760
• UniProt ID: P12314