Recombinant Human EXO5 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EXO5-6115H
• Product Overview: DEM1 MS Standard C13 and N15-labeled recombinant protein (NP_073611) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 41.9 kDa
• AA Sequence: MAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSSTLAPQVKKAKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EXO5 exonuclease 5 [ Homo sapiens (human) ]
• Official Symbol: EXO5
• Synonyms: EXO5; exonuclease 5; DEM1; Exo V; hExo5; C1orf176; exonuclease V; defects in morphology 1 homolog; defects in morphology protein 1 homolog; probable exonuclease V
• Gene ID: 64789
• mRNA Refseq: NM_022774
• Protein Refseq: NP_073611
• MIM: 618601
• UniProt ID: Q9H790