Recombinant Human eukaryotic translation initiation factor 4 gamma, 1 Protein, His&Flag&StrepII tagged

• Cat.No.: EIF4G1-195H
• Product Overview: Recombinant human EIF4G1 protein (Q04637-1) (Thr 330-Gln 600) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 330-600aa
• Molecular Mass: 35.2 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: EIF4G1 eukaryotic translation initiation factor 4 gamma, 1 [ Homo sapiens (human) ]
• Official Symbol: EIF4G1
• Synonyms: EIF4G1; eukaryotic translation initiation factor 4 gamma, 1; EIF4F, EIF4G; eukaryotic translation initiation factor 4 gamma 1; p220; EIF4-gamma; eIF-4-gamma 1; eucaryotic translation initiation factor 4G; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; DKFZp686A1451
• Gene ID: 1981
• mRNA Refseq: NM_001194946
• Protein Refseq: NP_001181875
• MIM: 600495
• UniProt ID: Q04637