Recombinant Human ERBB3 protein(Met1-Thr643), hFc-tagged

• Cat.No.: ERBB3-44H
• Product Overview: Recombinant Human ErbB3 (NP_001973.2)(Met 1-Thr 643) was expressed in HEK293 with the C-terminal fused Fc region of Human IgG1.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-643 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized HER3/ERBB3 Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind NRG1 Beta 1 Protein, Human, Recombinant, Biotinylated(EGF Domain, Avi&Fc Tag), the EC50 is 220-1200 ng/mL.
• Molecular Mass: The mature recombinant human ErbB3/Fc chimera is a disulfide-linked homodimeric protein after the removal of signal peptide. The monomer consists of 862 amino acids and has a calculated molecular mass of 95.4 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Homo sapiens ]
• Official Symbol: ERBB3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; MGC88033
• Gene ID: 2065
• mRNA Refseq: NM_001005915
• Protein Refseq: NP_001005915
• MIM: 190151
• UniProt ID: P21860