Recombinant Human EP300 Protein (bromodomain, 1041-1161), N-His and C-Flag-tagged

• Cat.No.: EP300-12H
• Product Overview: The peptide corresponding to amino acids 1041 - 1161 that contains the bromodomain sequences of EP300 (accession number NM_001429.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.1 kDa. It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102. The recombinant protein is >90% pure by SDS-PAGE.

Specification

• Description: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
• Source: E. coli
• Species: Human
• Tag: His&Flag
• Molecular Mass: 19.1 kDa
• Protein length: 1041-1161
• Applications: Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
• Notes: This product is for research use only and is not for use in diagnostic procedures.
• Stability: This product is guaranteed for 6 months from date of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 5 μg/μL
• Storage Buffer: Recombinant p300 (1041-1161) protein expressed in E. coli at a concentration of 5 μg/μL in 25 mM Tris pH 7.4, 150 mM NaCl, 5% Glycerol.

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens (human) ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; p300; KAT3B; MKHK2; RSTS2; histone acetyltransferase p300; E1A-associated protein p300; E1A-binding protein, 300kD; histone butyryltransferase p300; histone crotonyltransferase p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; EC 2.3.1.48
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q7Z6C1