Recombinant Human ENO1 Protein, 1-434aa

• Cat.No.: ENO1-1814H
• Product Overview: Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma.
• Source: E. coli
• Species: Human
• Protein length: 1-434aa
• Form: Liquid
• Bio-activity: > 20,000 pmol/min/μg, and was obtained by measuring the decrease of NAD in absorbance at 340 nm resulting from NADH at pH 6.5 at 37 centigrade.
• Molecular Mass: 47.1 kDa (434aa) confirmed by MALDI-TOF
• AA Sequence: MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
• References: 1. Das R., et al. (2009) Blood. 113(22):5371-2.
  2. ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.

Gene Information

• Gene Name: ENO1 enolase 1, (alpha) [ Homo sapiens (human) ]
• Official Symbol: ENO1
• Synonyms: ENO1; enolase 1, (alpha); ENO1L1, MPB1; alpha-enolase; c-myc promoter-binding protein-1; MBP 1; PPH; alpha-enolase; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase; NNE; MPB1; ENO1L1
• Gene ID: 2023
• mRNA Refseq: NM_001428
• Protein Refseq: NP_001419.1
• MIM: 172430
• UniProt ID: P06733