Recombinant Human Endonuclease G, His-tagged, strepII-tagged

• Cat.No.: ENDOG-388H
• Product Overview: Recombinant human ENDOG (aa. 1-140) produced inE.coliis a 15 kDa protein. It was fused to His-tag and strepII-tag at N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His&Strep II
• Protein Length: 1-140 a.a.
• Description: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
• Purity: 95%.
• Application: This recombinant protein can be used for WB, ELISA, MS.
• Tested Application: E, WB, MS.
• Buffer: 1X PBS containing 0.1% SDS and 0.02% NaN3.
• Species Reactivity: n/a.
• Storage: Store at -70°C with any protein, exposing EndoG recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Pathways: Apoptosis

Gene Information

• Gene Name: ENDOG endonuclease G [ Homo sapiens ]
• Synonyms: endonuclease G; FLJ27463; ENDOG; EC 3.1.30.-; OTTHUMP00000022301; Endo G
• Gene ID: 2021
• mRNA Refseq: NM_004435
• Protein Refseq: NP_004426
• MIM: 600440
• UniProt ID: Q14249
• Chromosome Location: 9q34.1
• Function: endonuclease activity; hydrolase activity; magnesium ion binding; manganese ion binding; nucleic acid binding