Recombinant Human EEF1D, His-tagged

• Cat.No.: EEF1D-3463H
• Product Overview: Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: AKR1D1-3463H
• Description: Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl
• Molecular Weight: 39.5 kDa (346aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AKR1D1 aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) [ Homo sapiens ]http://www.ncbi.nlm.nih.gov/sites/entrez
• Synonyms: delta(4)-3-oxosteroid 5-beta-reductase; delta(4)-3-ketosteroid 5-beta-reductase; steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1); EC 1.3.1.3; OTTHUMP00000208645; AKR1D1; CBAS2; SRD5B1; 3o5bred; 3-oxo-5-beta-steroid 4-dehydrogenase
• Gene ID: 6718http://www.ncbi.nlm.nih.gov/sites/entrez
• mRNA Refseq: NM_001190906http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NM_002046
• Protein Refseq: NP_001177835http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_002037
• MIM: 604741http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=138400
• UniProt ID: P51857
• Chromosome Location: 7q32-q33http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?direct=on&idtype=gene&id=9470
• Pathway: Metabolic pathways; Steroid hormone biosynthesis; Primary bile acid biosynthesis; Metabolism of lipids and lipoproteins
• Function: 3-oxo-5-beta-steroid 4-dehydrogenase activity; aldo-keto reductase activity; delta4-3-oxosteroid 5beta-reductase activity; oxidoreductase activity; steroid binding