Recombinant Human EEF1A1 Protein, Myc/DDK-tagged

• Cat.No.: EEF1A1-001H
• Product Overview: Recombinant protein of human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
• Molecular Mass: 50 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Applications: ELISA capture for autoantibodies
  Biolayer interferometry (BLI) assay
  Binding assay
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: EEF1A1 eukaryotic translation elongation factor 1 alpha 1 [ Homo sapiens (human) ]
• Official Symbol: EEF1A1
• Synonyms: EEF1A1; eukaryotic translation elongation factor 1 alpha 1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; elongation factor 1-alpha 1; CTCL tumor antigen; EF-1-alpha-1; EF1a-like protein; cervical cancer suppressor 3; elongation factor 1 alpha subunit; elongation factor Tu; epididymis secretory sperm binding protein; eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; leukocyte receptor cluster (LRC) member 7; leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; translation elongation factor 1 alpha 1-like 14
• Gene ID: 1915
• mRNA Refseq: NM_001402
• Protein Refseq: NP_001393
• MIM: 130590
• UniProt ID: P68104