Recombinant Human EED protein(Met1-Arg441), His&GST-tagged

• Cat.No.: EED-6921H
• Product Overview: Recombinant Human EED (O75530-1) (Met 1-Arg 441) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-441 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 2mM GSH, 10% gly Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human EED/GST chimera consists of 678 amino acids and has a calculated molecular mass of 78 kDa. It migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiationrelated genes to promote self-renewal (2023)

Gene Information

• Gene Name: EED embryonic ectoderm development [ Homo sapiens ]
• Official Symbol: EED
• Synonyms: EED; embryonic ectoderm development; polycomb protein EED; HEED; WAIT 1; WD protein associating with integrin cytoplasmic tails 1; WAIT1
• Gene ID: 8726
• mRNA Refseq: NM_003797
• Protein Refseq: NP_003788
• MIM: 605984
• UniProt ID: O75530