Recombinant Human DSG3 protein, His-tagged

• Cat.No.: DSG3-4070H
• Product Overview: Recombinant Human DSG3 protein(P32926)(Glu50-Arg615), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Glu50-Arg615
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human DSG3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DSG3 Antibody, hFc Tag with the EC50 4.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 64 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DSG3 desmoglein 3 [ Homo sapiens ]
• Official Symbol: DSG3
• Synonyms: DSG3; desmoglein 3; desmoglein 3 (pemphigus vulgaris antigen); desmoglein-3; CDHF6; pemphigus vulgaris antigen; cadherin family member 6; 130-kD pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen; PVA; DKFZp686P23184
• Gene ID: 1830
• mRNA Refseq: NM_001944
• Protein Refseq: NP_001935
• MIM: 169615
• UniProt ID: P32926