Recombinant Human DSG3 protein, His-tagged

• Cat.No.: DSG3-4068H
• Product Overview: Recombinant Human DSG3 protein(P32926)(50-615aa), fused with C-terminal His tag, was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: 50-615aa
• Tag: C-His
• Form: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 4.552-5.720 ng/mL.
• Molecular Mass: 63.8 kDa
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.
• AA Sequence: EWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR

Gene Information

• Gene Name: DSG3 desmoglein 3 [ Homo sapiens ]
• Official Symbol: DSG3
• Synonyms: DSG3; desmoglein 3; desmoglein 3 (pemphigus vulgaris antigen); desmoglein-3; CDHF6; pemphigus vulgaris antigen; cadherin family member 6; 130-kD pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen; PVA; DKFZp686P23184
• Gene ID: 1830
• mRNA Refseq: NM_001944
• Protein Refseq: NP_001935
• MIM: 169615
• UniProt ID: P32926