Recombinant Human DPP10 protein(Leu56-Glu796), His-tagged

• Cat.No.: DPP10-943H
• Product Overview: Recombinant Human DPP10 isoform 1 (Q8N608-1) (Leu 56-Glu 796) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 56-796 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human DPP10 consists of 760 amino acids and has a calculated molecular mass of 87.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhDPP10 is approximately 90-110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DPP10 dipeptidyl-peptidase 10 (non-functional) [ Homo sapiens ]
• Official Symbol: DPP10
• Synonyms: DPP10; dipeptidyl-peptidase 10 (non-functional); dipeptidyl peptidase 10 , dipeptidylpeptidase 10; inactive dipeptidyl peptidase 10; DPRP3; DPP X; DPRP-3; dipeptidyl peptidase X; dipeptidyl peptidase 10; dipeptidyl peptidase like protein 2; dipeptidyl peptidase-like protein 2; dipeptidyl peptidase IV-related protein 3; DPL2; DPPY
• Gene ID: 57628
• mRNA Refseq: NM_001004360
• Protein Refseq: NP_001004360
• MIM: 608209
• UniProt ID: Q8N608

Case Study

Case 1: Zhang Y, et al. Dis Model Mech. 2018

This study validates DPP10 as an asthma susceptibility gene, linking a β-propeller mutation (Val→Asp) to heightened airway resistance post-allergen challenge in mice. Dpp10 mutant mice exhibited elevated serum IgE, eosinophil infiltration, and airway hyperresponsiveness after house dust mite (HDM) exposure. Human asthmatic tissues and HDM-treated mice showed altered DPP10 expression in airway epithelial cells, with knockdown experiments revealing disrupted cytokine responses. These findings functionally connect DPP10 variants to allergic inflammation, supporting genetic associations and highlighting its role in epithelial-driven asthma pathogenesis.

Fig1. Gel comparing the genotyping results from wild-type (left lane) and heterozygous (right lane) Dpp145D littermates.

Fig2. A concentration-dependent reduction in DPP10 protein expression was observed after 24 h in BEAS-2B cells following siRNA knockdown.

Case 2: Jerng HH, et al. Mol Cell Neurosci. 2007

This study reveals that DPP10 splice variants differentially regulate Kv4 channel complexes governing neuronal A-type currents (ISA). While all DPP10 isoforms accelerate gating, the DPP10a variant uniquely drives voltage-dependent fast inactivation, dominating in cortical circuits. Regional expression analysis shows DPP10a is enriched in the cortex, whereas DPP10c/d are broadly distributed. These isoform-specific effects and spatial expression patterns suggest DPP10a underpins rapid ISA inactivation in cortical neurons, highlighting splice variation as a mechanism for fine-tuning excitability across brain regions.

Fig1. DPP10a, but not DPP10c or DPP10d, introduces a distinct rapid inactivation to Kv4.2-mediated current at more depolarized potentials.

Fig2. Co-expression of DPP10 variants results in differential leftward shifts in the conductance-voltage relationship.

Application

1. Recombinant DPP10 Protein: Unlocking Insights into Neurological and Immune Disorders Recombinant DPP10 (dipeptidyl peptidase-like protein 10) is gaining prominence for its role in regulating voltage-gated potassium channels and neuronal excitability, with implications in epilepsy, neuropathic pain, and neurodegenerative diseases. Studies highlight its interaction with Kv4 channels, which modulate action potential dynamics in neurons. Aberrant DPP10 expression is linked to asthma and autoimmune disorders, where it influences T-cell activation and inflammatory responses. Researchers employ recombinant DPP10 to dissect these mechanisms, screen ion channel-targeting drugs, and develop biomarkers for disease stratification, particularly in treatment-resistant neurological conditions. 2. Biotechnological Advancements and Therapeutic Potential High-purity recombinant DPP10 is produced using mammalian expression systems, ensuring proper post-translational modifications critical for functional studies. Its application extends to structural biology, enabling cryo-EM studies of potassium channel complexes, and to drug discovery platforms identifying modulators for channelopathies. In asthma research, recombinant DPP10 aids in mapping its immunomodulatory effects on airway hyperresponsiveness. Additionally, it serves as a reference standard in diagnostic assays to detect autoantibodies in autoimmune encephalitis, enhancing early intervention strategies. 3. Future Directions in Precision Medicine and Beyond Emerging research explores DPP10’s role in cancer metastasis via ion channel-mediated cell migration mechanisms. CRISPR-Cas9-edited models combined with recombinant DPP10 are clarifying its tissue-specific functions, while nanoparticle-based delivery systems aim to target neurological pathways in vivo. Collaborations between academia and biotech firms focus on developing DPP10-based biologics for rare channelopathies and inflammatory diseases, aligning with growing demand for personalized therapies. As omics data reveal novel DPP10-interacting proteins, its utility in multi-target drug design and biomarker panels continues to expand.