Recombinant Human Dopa Decarboxylase, His-tagged

• Cat.No.: DDC-141H
• Product Overview: Recombinant human Dopa decarboxylase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 56.4 kDa (503aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-480 aa
• Description: Dopa decarboxylase, also known as DDC, is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is a protein implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Dopa decarboxylase is found in different areas of the brain and is particularly abundant in basal ganglia.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH TRSMNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: dopa decarboxylase [ Homo sapiens ]
• Synonyms: DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); AADC; L-Dopa decarboxylase; aromatic L-amino acid decarboxylase; EC4.1.1.28;DOPA decarboxylase
• Gene ID: 1644
• mRNA Refseq: NM_ 000790
• Protein Refseq: NP_ 000781
• MIM: 107930
• UniProt ID: P20711
• Chromosome Location: 7p12.2
• Pathway: Alkaloid biosynthesis I; Histidine metabolism; Phenylalanine metabolism; Tryptophan metabolism; Tyrosine metabolism; Synaptic Transmission
• Function: aromatic-L-amino-acid decarboxylase activity; lyase activity; protein binding; pyridoxal phosphate binding