Recombinant Human DNAJB11, His-tagged

• Cat.No.: DNAJB11-3490H
• Product Overview: Recombinant human DNAJB11 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DNAJB11 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It serves as a co-chaperone for HSPA5 and binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 40.5 kDa (357aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGRDFYKILG VPRSASIKDI KKAYRKLALQ LHPDRNPDDP QAQEKFQDLG AAYEVLSDSE KRKQYDTYGE EGLKDGHQSS HGDIFSHFFG DFGFMFGGTP RQQDRNIPRG SDIIVDLEVT LEEVYAGNFV EVVRNKPVAR QAPGKRKCNC RQEMRTTQLG PGRFQMTQEV VCDECPNVKL VNEERTLEVE IEPGVRDGME YPFIGEGEPH VDGEPGDLRF RIKVVKHPIF ERRGDDLYTN VTISLVESLV GFEMDITHLD GHKVHISRDK ITRPGAKLWK KGEGLPNFDN NNIKGSLIIT FDVDFPKEQL TEEAREGIKQ LLKQGSVQKV YNGLQGY
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DNAJB11 DnaJ (Hsp40) homolog, subfamily B,member 11 [ Homo sapiens ]
• Synonyms: DNAJB11; DJ9; EDJ; ERj3; HEDJ; hDj9; ABBP2; ERdj3; ABBP-2; UNQ537; PRO1080; dnaJ homolog subfamily B member 11; ERj3p; hDj-9; OTTHUMP00000210171; human DnaJ protein 9; dnaJ protein homolog 9; APOBEC1-binding protein 2; PWP1-interacting protein 4; ER-associated DNAJ protein 3; ER-associated Hsp40 co-chaperone
• Gene ID: 51726
• mRNA Refseq: NM_016306
• Protein Refseq: NP_057390
• MIM: 611341
• UniProt ID: Q9UBS4
• Chromosome Location: 3q27.3
• Function: heat shock protein binding; protein binding; unfolded protein binding