Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 1, His-tagged

• Cat.No.: DNAJB1-4996H
• Product Overview: Recombinant human HSP40, his-tagged, is overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris, pH 8.0, containing 2mM DTT, 0.2mM PMSF, and 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GW
• Molecular Mass: 40.2 kDa (360aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]
• Official Symbol: DNAJB1
• Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hdj1; Sis1; HSPF1; Hsp40; DNAJ1; HDJ-1; HDJ1; DnaJ protein homolog 1; Heat shock protein 40; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hsp40 (heat shock protein 40 kD)
• Gene ID: 3337
• mRNA Refseq: NM_006145
• Protein Refseq: NP_006136
• MIM: 604572
• UniProt ID: P25685
• Chromosome Location: 19p13.2
• Function: heat shock protein binding; unfolded protein binding